|
| Status |
Public on Nov 10, 2014 |
| Title |
OsEREBP1 over expressed transgenic rice plant, biol rep. 2 |
| Sample type |
RNA |
| |
|
| Source name |
20 days old 4-3-1 rice seedlings
|
| Organism |
Oryza sativa |
| Characteristics |
genotype/variation: OsEREBP1 over expressed transgenic rice plant
line: 4-3-1
age: 20 day-old seedlings
|
| Treatment protocol |
OsEREBP1 transcription factor over expressed transgenic plant comparison with non transgenic control
|
| Growth protocol |
The seeds from non transgenic control rice plants (Kitaki) and OsEREBP1 over expressed transgenic rice plants (4-3-1) were germinated in petri plates containing moistened filter paper. Three days old seedlings were transferred to plastic pots containing soil and were grown for 15 days in a green house. Two days before the actual treatment, the pots containing the rice seedlings were transferred to a growth chamber (28oC; 80% relative humidity with 12/12h light/dark cycle)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10-15 young leaves from Kitaki and 4-3-1 rice plants were harvested and subjected to total RNA isolation using Trizol (Invitrogen)
|
| Label |
Biotin
|
| Label protocol |
150ng of total RNA was converted into biotinylated aRNA using Genechip 3' IVT Express Kit as per instructions of the manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
The biotinylated aRNAs were fragmented and 15µg of this sample was hybridized to Gene Chip Rice Genome Array for 16h at 45oC and 60rpm. The hybridized gene chips were subjected to washing and staining protocol using an Affymetrix fluidics station 450
|
| Scan protocol |
The gene chips were scanned on an Affymetrix Scanner 3000
|
| Description |
Expression profile of OsEREBP1 over expressed transgenic rice plant
|
| Data processing |
The CEL files generated by the Gene Chip Operating System (Affymetrix) were subjected to GeneSpring 12.6 - GX - PA (Agilent Technology) for further analysis. The GC-RMA algorithm was used for data normalization
|
| |
|
| Submission date |
Nov 10, 2014 |
| Last update date |
Nov 10, 2014 |
| Contact name |
Rajeshwari Ramanan |
| E-mail(s) |
[email protected]
|
| Phone |
+919848017798
|
| Organization name |
Centre for Cellular and molecular biology
|
| Department |
Plant Science
|
| Lab |
E-207
|
| Street address |
Uppal Road
|
| City |
Hyderabad |
| State/province |
Andhra Pradesh |
| ZIP/Postal code |
500007 |
| Country |
India |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE46173 |
Transcriptional profiles of non-transgenic and OsEREBP1 over-expressed transgenic plants |
|
