|
| Status |
Public on Jan 20, 2015 |
| Title |
LTN013-2, aza-dc+TSA Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis seedlings were cold treated at 0 °C for 24 h following both aza and TSA treatment
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia
developmental stage: seedlings
|
| Treatment protocol |
Arabidopsis seedlings were cold treated at 0 °C for 24 h in growth chamber (16-h day length at 70% relative humidity).
|
| Growth protocol |
Seeds were sterilized with 70% ethanol, 0.05% Triton X-100 for 10 min and then washed with 95% ethanol, 0.05% Triton X-100 for 10 min, with 95% ethanol was for 10 min. A final washed with ddH2O for 3times, put into ddH2O in 4°C 48h. Seeds were sown on deep Petri plates with semisolid MS germination medium (Mock), added aza-dC and/or TSA. All seedlings kept in a growth chamber (16 h light and 8 h darkness, 70% relative humidity, 23 °C) for 16 days before being harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted using RNA purification kit (Norgen, Canada) following the manufacturer’s manual.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization, washing, and staining were performed according to the Affymetrix manufacturer’s Instructions.
|
| Scan protocol |
Microarrays were scanned using a ScanArray 4000 Microarray Acquisition System.
|
| Description |
Gene expression data from Arabidopsis seedlings were cold treated at 0 °C for 24 h following both aza and TSA treatment
|
| Data processing |
Microarray data were extracted from scanned GeneChip images and analyzed by use of Microarray Suite version 5.0.1 (Affymetrix) and BioConductor (http://www.bioconductor.org/).
|
| |
|
| Submission date |
Nov 10, 2014 |
| Last update date |
Jan 20, 2015 |
| Contact name |
Yuan Song |
| E-mail(s) |
[email protected]
|
| Organization name |
Lanzhou University
|
| Department |
the school of life science
|
| Street address |
the South of Tianshui Road
|
| City |
Lanzhou |
| State/province |
Gansu |
| ZIP/Postal code |
730000 |
| Country |
China |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE63131 |
Expression data from the Arabidopsis with freezing treatment following the inhibitors of DNA methylation and histone deacetylation |
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