|
| Status |
Public on May 16, 2007 |
| Title |
MycFlag-G1-arrest-t150-rep2 |
| Sample type |
other |
| |
|
| Channel 1 |
| Source name |
Myc IP 150 minutes rep2
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain: MDY510
|
| Treatment protocol |
Cells arrested with 4μg/mL alpha factor for 3 hours, collected by filtration and resuspended in YP+2% galactose.
|
| Growth protocol |
MDY510 cells were grown in YP + 2% raffinose
|
| Extracted molecule |
other |
| Extraction protocol |
IPed using anti-Myc Ab (Sigma; monoclonal). Amplified with a starting amount of up to 75 ng for ChIP samples, using the DNA linear amplification
|
| Label |
Cy5
|
| Label protocol |
3 μg of aRNA produced from the linear amplification were used to label probe
|
| |
|
| Channel 2 |
| Source name |
Flag IP 150 minutes rep2
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain: MDY510
|
| Treatment protocol |
Cells arrested with 4μg/mL alpha factor for 3 hours, collected by filtration and resuspended in YP+2% galactose.
|
| Growth protocol |
MDY510 cells were grown in YP + 2% raffinose
|
| Extracted molecule |
other |
| Extraction protocol |
IPed using anti-Flag Ab (Sigma; monoclonal). Amplified with a starting amount of up to 75 ng for ChIP samples, using the DNA linear amplification
|
| Label |
Cy3
|
| Label protocol |
3 μg of aRNA produced from the linear amplification were used to label probe
|
| |
|
| |
| Hybridization protocol |
Labeled probes were hybridized onto a yeast tiled oligonucleotide microarray [Yuan et al., Science 309, 626 (2005)] at 65°C for 16 hours and washed as described on www.microarrays.org.
|
| Scan protocol |
The arrays were scanned at 5 micron resolution with an Axon Laboratories GenePix 4000B scanner running GenePix 5.1. Image analysis and data normalization were performed as previously described [Liu et al., PLoS Biol 3, e328 (2005)].
|
| Description |
High-resolution Myc/Flag rations (using IP for Gal-driven Flag-H3 and constitutive Myc-H3). 150min after Gal induction.
|
| Data processing |
log2(Cy5/Cy3)
|
| |
|
| Submission date |
Jan 05, 2007 |
| Last update date |
May 16, 2007 |
| Contact name |
Tommy Kaplan |
| E-mail(s) |
[email protected]
|
| Organization name |
Hebrew University
|
| Department |
School of Computer Science and Engineering
|
| Street address |
Givat Ram Campus
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL2625 |
| Series (2) |
| GSE6665 |
Ratios for G1 arrested cells (Printed array) |
| GSE6680 |
Dynamics of replication-independent histone turnover in budding yeast |
|
