|
| Status |
Public on Jan 20, 2007 |
| Title |
Normal mucosa 20 |
| Sample type |
RNA |
| |
|
| Source name |
human head and neck normal mucosa
|
| Organism |
Homo sapiens |
| Characteristics |
tissue sample from Bellevue Hospital, NY
|
| Treatment protocol |
normal tissue collected during removal of head and neck cancer, snap frozen with lq N2 in operating room, stored at -80 until RNA isolation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from `10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human U95A.v2 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data of human normal head and neck mucosa.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. CEL files were normalized with RMA and signal intensity of probe sets were computed with GeneTraffic v3.2 (Iobion Informatics).
|
| |
|
| Submission date |
Jan 03, 2007 |
| Last update date |
Mar 16, 2009 |
| Contact name |
Fang-An Chen |
| E-mail(s) |
[email protected]
|
| Phone |
212-998-9564
|
| Fax |
212-995-4087
|
| Organization name |
New York Univ. College of Dentistry
|
| Department |
Oral and Maxillifacial surgery
|
| Street address |
345E 24th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10010 |
| Country |
USA |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE6631 |
Expression data from head and neck squamous cell carcinoma |
|
