|
| Status |
Public on Nov 06, 2014 |
| Title |
Bonemarrow_KO_Untreated_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow derived eosinophil
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone marrow
cell type: eosinophils
treatment: Untreated
genotype: KO (Dusp5-/-)
|
| Treatment protocol |
Day 12 bone marrow derived eosinophils from WT and KO were left untreated (media only) or IL-33 (25ng/ml) stimulated for 4 hours.
|
| Growth protocol |
Bone marrow derived eosinophils from WT and KO mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Treated cells were washed with ice cold PBS and cell pellets were directly lysed in RLT buffer (Qiagen) followed by immediate RNA isolation (RNEasy plus mini kit with genomic DNA eliminator columns).
|
| Label |
biotin
|
| Label protocol |
Preparation of cRNA was done using Affymetrix 3'IVT Express kit and protocol provided by Affymetrix. Briefly, 0.5 ug of total RNA was converted into double-stranded cDNA and used to generate biotin labeled cRNA which was then purified.
|
| |
|
| Hybridization protocol |
12.5 micrograms of cRNA was fragmented and hybridized to Mouse Genome 430 2.0 arrays at 45°C for 19 hours in a rotisserie oven set at 60 rpm.
|
| Scan protocol |
Arrays were washed and stained using Affymrtrix HWS kit using their Fluidics station and then scanned on GeneChip scanner 3000.
|
| Description |
GeneExpressionData_Bonemarrow_KO_Untreated_rep2
|
| Data processing |
Data analysis was performed using the Affymetrix GeneChip operating system and analysis software.
|
| |
|
| Submission date |
Nov 05, 2014 |
| Last update date |
Nov 06, 2014 |
| Contact name |
Derek A Holmes |
| E-mail(s) |
[email protected]
|
| Phone |
+1 650 467 0939
|
| Organization name |
Genentech Inc
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE62999 |
Expression data from study on IL33 signaling in Dusp5 deficient eosinophils |
|
