|
| Status |
Public on Sep 28, 2016 |
| Title |
Tan_Female_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Blood
|
| Organism |
Ovis aries |
| Characteristics |
breed: Tan sheep
gender: Female
tissue type: Venous blood
age: post natal day 343
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Blood genomic DNA was isolated according to the manufacturer’s protocol of TIANamp Blood DNA Kit (DP318, TIANGEN, Beijing)
Adaptor-ligated DNA was used for MeDIP enrichment based on the manufacturer’s recommendation of Magnetic Methylated DNA Immunoprecipitation kit (Diagenod, Liège, Belgium), MeDIP libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
MeDIP-Seq |
| Library source |
genomic |
| Library selection |
5-methylcytidine antibody |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Illumina software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to Ovis aries Oar_v3.1 whole genome using SOAP aligner v 2.21 with parameters -s 34 -p 4 -v 2
Peaks were called using MACs 1.4.0
Genome_build: Ovis aries Oar_v3.1
Supplementary_files_format_and_content: wig files were generated using MACs.1.4.0
|
| |
|
| Submission date |
Oct 15, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiaxue Cao |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
|
| Street address |
Yuanmingyuan West Road 2#
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL15670 |
| Series (1) |
| GSE62345 |
DNA methylation Landscape of body size variation in sheep |
|
| Relations |
| BioSample |
SAMN03108566 |
| SRA |
SRX732930 |
