|
| Status |
Public on Sep 28, 2014 |
| Title |
1013_day_0 |
| Sample type |
RNA |
| |
|
| Source name |
Human whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
severity: Severe
virus type: pdm2009/H1N1
age: 40
timepoint: day_0
tissue: Whole Blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from whole blood drawn in pax-gene vacutainer tubes by PAX-gene RNA kit (Qiagen). Quality control was performed with NanoDrop 1000 instrument
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were pared with the Illumina TotalP RNA Amplification Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
All datasets were normalized by using R (http://www.bioconductor.org). The raw data was log10 transformed before Z score transformation was performed [10]. The Z score was calculated for each sample by subtracting the overall mean gene intensity from the raw intensity signal for each gene. After that, this data was divided by the standard deviation of all of the measured intensities
|
| |
|
| Submission date |
Sep 26, 2014 |
| Last update date |
Sep 28, 2014 |
| Contact name |
Ahmad Nazri Mohamed Naim |
| E-mail(s) |
[email protected]
|
| Phone |
65-98547654
|
| Organization name |
Genome institute of Singapore
|
| Department |
Infectious disease
|
| Lab |
lab 1
|
| Street address |
60 Biopolis Street, Genome, #02-01
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE61821 |
Patient-based transcriptome-wide analysis identify interferon and ubiquination pathways as potential predictors of Influenza A disease severity |
|
