|
| Status |
Public on Jan 01, 2015 |
| Title |
knockout biological replicate 4 |
| Sample type |
RNA |
| |
|
| Source name |
Epcam(+) cells from E18.5 lungs
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: embryo
age: E18.5
tissue: lung
cell type: Epcam(+) epithelial cells
genotype/variation: Mst1/2 CKO
|
| Treatment protocol |
Lungs dissected from E18.5 embryos were mechanically/enzymatically dissociated to generate single cell suspension. Epcam(+) cells were isolated using magnetic microbeads and an AutoMACS Pro Separator (Miltenyi Biotec)
|
| Growth protocol |
Timed matings were established with the presence of a copulation plug representing day 0.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from Epcam(+) cells using a RNeasy Micro Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Based on Affymetrix protocols for biotin labeling
|
| |
|
| Hybridization protocol |
Based on Affymetrix protocols
|
| Scan protocol |
Based on Affymetrix protocols
|
| Description |
Mst1/2 CKO_10P
|
| Data processing |
Quantile normalized gene level expression values from Gene Spring MoGene-1_0-st-v1.r4.pgf
|
| |
|
| Submission date |
Sep 19, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Alexander Lange |
| E-mail(s) |
[email protected]
|
| Organization name |
Cincinnati Children's Hospital Reseach Foundation
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE61582 |
Microarray of Mst1/2 deleted epithelial cells from E18.5 mouse lungs |
| GSE61628 |
Mst1/2-Yap in lung epithelial progenitor cells |
|
