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Sample GSM150327 Query DataSets for GSM150327
Status Public on Dec 15, 2006
Title rab3a+/+ hippocampus hrw2 B
Sample type RNA
 
Source name hippocampus
Organism Mus musculus
Characteristics rab3a+/+ hippocampus
Extracted molecule total RNA
Extraction protocol Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description hippocampus from rab3a+/+ mouse
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 14, 2006
Last update date Dec 14, 2006
Contact name Maja Bucan
E-mail(s) [email protected]
Organization name UPENN
Street address 415 Curie Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL340
Series (1)
GSE6527 Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 223.2 P 0.020022
AFFX-BioB-M_at 211 P 0.000509
AFFX-BioB-3_at 150.1 P 0.00762
AFFX-BioC-5_at 548.9 P 0.000297
AFFX-BioC-3_at 419.6 P 0.000195
AFFX-BioDn-5_at 456.2 P 0.000052
AFFX-BioDn-3_at 2943 P 0.000147
AFFX-CreX-5_at 5340.7 P 0.000044
AFFX-CreX-3_at 10852.1 P 0.000044
AFFX-DapX-5_at 34.8 A 0.216524
AFFX-DapX-M_at 33.1 A 0.300606
AFFX-DapX-3_at 4.3 A 0.979978
AFFX-LysX-5_at 11.8 A 0.659339
AFFX-LysX-M_at 10.9 A 0.760937
AFFX-LysX-3_at 34.4 M 0.062929
AFFX-PheX-5_at 3.3 A 0.953518
AFFX-PheX-M_at 1.2 A 0.969024
AFFX-PheX-3_at 16.3 A 0.672921
AFFX-ThrX-5_at 8.2 A 0.883887
AFFX-ThrX-M_at 2.4 A 0.883887

Total number of rows: 22575

Table truncated, full table size 598 Kbytes.




Supplementary file Size Download File type/resource
GSM150327.CEL.gz 3.3 Mb (ftp)(http) CEL

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