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Sample GSM150302

Query DataSets for GSM150302

Status

Public on Dec 15, 2006

Title

earlybird wildtype cortex cew1 B

Sample type

RNA

Source name

cortex

Organism

Mus musculus

Characteristics

earlybird wildtype cortex

Extracted molecule

total RNA

Extraction protocol

Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

cortex from wild type littermate of earlybird mutant mouse

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Dec 14, 2006

Last update date

Dec 14, 2006

Contact name

Maja Bucan

E-mail(s)

[email protected]

Organization name

UPENN

Street address

415 Curie Blvd

City

Philadelphia

State/province

ZIP/Postal code

19104

Country

USA

Platform ID

GPL340

Series (1)

GSE6527

Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	229.4	P	0.01667
AFFX-BioB-M_at	228.8	P	0.00039
AFFX-BioB-3_at	143.7	P	0.002023
AFFX-BioC-5_at	484.5	P	0.00141
AFFX-BioC-3_at	447.1	P	0.00011
AFFX-BioDn-5_at	504.8	P	0.00006
AFFX-BioDn-3_at	2821.6	P	0.000147
AFFX-CreX-5_at	5830.4	P	0.000044
AFFX-CreX-3_at	9649.2	P	0.000044
AFFX-DapX-5_at	6.7	A	0.574038
AFFX-DapX-M_at	50.8	A	0.382599
AFFX-DapX-3_at	12.8	A	0.794268
AFFX-LysX-5_at	1.7	A	0.995983
AFFX-LysX-M_at	39.7	A	0.617401
AFFX-LysX-3_at	41.3	A	0.195266
AFFX-PheX-5_at	18.7	A	0.749204
AFFX-PheX-M_at	11.9	A	0.672921
AFFX-PheX-3_at	52	A	0.48511
AFFX-ThrX-5_at	8	A	0.916408
AFFX-ThrX-M_at	3.7	A	0.949771

Total number of rows: 22575

Table truncated, full table size 598 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM150302.CEL.gz	3.3 Mb	(ftp)(http)	CEL