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Sample GSM150284

Query DataSets for GSM150284

Status

Public on Dec 15, 2006

Title

earlybird wildtype hippocampus hew1A

Sample type

RNA

Source name

hippocampus

Organism

Mus musculus

Characteristics

earlybird wildtype hippocampus

Extracted molecule

total RNA

Extraction protocol

Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

hippocampus from wild type littermate of earlybird mutant mouse

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Dec 14, 2006

Last update date

Dec 14, 2006

Contact name

Maja Bucan

E-mail(s)

[email protected]

Organization name

UPENN

Street address

415 Curie Blvd

City

Philadelphia

State/province

ZIP/Postal code

19104

Country

USA

Platform ID

GPL339

Series (1)

GSE6527

Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	134.4	P	0.012547
AFFX-BioB-M_at	200.3	P	0.000225
AFFX-BioB-3_at	88.7	P	0.00141
AFFX-BioC-5_at	360	P	0.00034
AFFX-BioC-3_at	229.9	P	0.00011
AFFX-BioDn-5_at	256.7	P	0.00006
AFFX-BioDn-3_at	1757.3	P	0.000095
AFFX-CreX-5_at	3494.6	P	0.000044
AFFX-CreX-3_at	5098.3	P	0.000044
AFFX-DapX-5_at	1.6	A	0.686277
AFFX-DapX-M_at	17.9	A	0.287743
AFFX-DapX-3_at	2	A	0.990663
AFFX-LysX-5_at	7.7	A	0.659339
AFFX-LysX-M_at	3	A	0.772364
AFFX-LysX-3_at	10.2	A	0.368438
AFFX-PheX-5_at	1.4	A	0.941556
AFFX-PheX-M_at	2.2	A	0.868639
AFFX-PheX-3_at	6.4	A	0.783476
AFFX-ThrX-5_at	3.7	A	0.916408
AFFX-ThrX-M_at	8.8	A	0.834139

Total number of rows: 22690

Table truncated, full table size 609 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM150284.CEL.gz	3.2 Mb	(ftp)(http)	CEL