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Sample GSM150270 Query DataSets for GSM150270
Status Public on Dec 15, 2006
Title earlybird mutant cortex cem2A
Sample type RNA
 
Source name cortex
Organism Mus musculus
Characteristics earlybird mutant cortex
Extracted molecule total RNA
Extraction protocol Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description cortex from earlybird mutant mouse
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 14, 2006
Last update date Dec 14, 2006
Contact name Maja Bucan
E-mail(s) [email protected]
Organization name UPENN
Street address 415 Curie Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL339
Series (1)
GSE6527 Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 111 P 0.023929
AFFX-BioB-M_at 157.4 P 0.000127
AFFX-BioB-3_at 69.1 P 0.006871
AFFX-BioC-5_at 293.4 P 0.00039
AFFX-BioC-3_at 183.5 P 0.000127
AFFX-BioDn-5_at 224.8 P 0.00006
AFFX-BioDn-3_at 1371.2 P 0.000225
AFFX-CreX-5_at 2350.1 P 0.000044
AFFX-CreX-3_at 3540.3 P 0.000044
AFFX-DapX-5_at 21.5 A 0.165861
AFFX-DapX-M_at 13.7 A 0.300606
AFFX-DapX-3_at 2.8 A 0.834139
AFFX-LysX-5_at 2.5 A 0.783476
AFFX-LysX-M_at 6.3 A 0.631562
AFFX-LysX-3_at 15.1 A 0.116113
AFFX-PheX-5_at 2.1 A 0.9273
AFFX-PheX-M_at 3.3 A 0.724854
AFFX-PheX-3_at 7 A 0.737173
AFFX-ThrX-5_at 5.2 A 0.860518
AFFX-ThrX-M_at 15.4 A 0.440646

Total number of rows: 22690

Table truncated, full table size 610 Kbytes.




Supplementary file Size Download File type/resource
GSM150270.CEL.gz 3.3 Mb (ftp)(http) CEL

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