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Sample GSM150268

Query DataSets for GSM150268

Status

Public on Dec 15, 2006

Title

earlybird wildtype cortex cew3A

Sample type

RNA

Source name

cortex

Organism

Mus musculus

Characteristics

earlybird wildtype cortex

Extracted molecule

total RNA

Extraction protocol

Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

cortex from wild type littermate of earlybird mutant mouse

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Dec 14, 2006

Last update date

Dec 14, 2006

Contact name

Maja Bucan

E-mail(s)

[email protected]

Organization name

UPENN

Street address

415 Curie Blvd

City

Philadelphia

State/province

ZIP/Postal code

19104

Country

USA

Platform ID

GPL339

Series (1)

GSE6527

Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	90.9	P	0.006871
AFFX-BioB-M_at	134.2	P	0.00039
AFFX-BioB-3_at	57.7	P	0.000446
AFFX-BioC-5_at	275.6	P	0.000258
AFFX-BioC-3_at	196.5	P	0.00007
AFFX-BioDn-5_at	249.8	P	0.00007
AFFX-BioDn-3_at	1285.6	P	0.000127
AFFX-CreX-5_at	2510.3	P	0.000044
AFFX-CreX-3_at	3919.8	P	0.000044
AFFX-DapX-5_at	2.1	A	0.749204
AFFX-DapX-M_at	13.4	A	0.175328
AFFX-DapX-3_at	2.1	A	0.969024
AFFX-LysX-5_at	1.2	A	0.937071
AFFX-LysX-M_at	36.3	A	0.262827
AFFX-LysX-3_at	8.7	A	0.672921
AFFX-PheX-5_at	1.2	A	0.945787
AFFX-PheX-M_at	2.2	A	0.876428
AFFX-PheX-3_at	13.7	A	0.574038
AFFX-ThrX-5_at	9.6	A	0.659339
AFFX-ThrX-M_at	12.8	A	0.354453

Total number of rows: 22690

Table truncated, full table size 610 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM150268.CEL.gz	3.3 Mb	(ftp)(http)	CEL