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Sample GSM150266

Query DataSets for GSM150266

Status

Public on Dec 15, 2006

Title

earlybird wildtype cortex cew1A

Sample type

RNA

Source name

cortex

Organism

Mus musculus

Characteristics

earlybird wildtype cortex

Extracted molecule

total RNA

Extraction protocol

Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

cortex from wild type littermate of earlybird mutant mouse

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Dec 14, 2006

Last update date

Dec 14, 2006

Contact name

Maja Bucan

E-mail(s)

[email protected]

Organization name

UPENN

Street address

415 Curie Blvd

City

Philadelphia

State/province

ZIP/Postal code

19104

Country

USA

Platform ID

GPL339

Series (1)

GSE6527

Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	102.4	P	0.026111
AFFX-BioB-M_at	81	P	0.000195
AFFX-BioB-3_at	44	P	0.006871
AFFX-BioC-5_at	247.3	P	0.000662
AFFX-BioC-3_at	176.4	P	0.00006
AFFX-BioDn-5_at	217.7	P	0.00007
AFFX-BioDn-3_at	1236.4	P	0.000169
AFFX-CreX-5_at	2318.9	P	0.000044
AFFX-CreX-3_at	4180.5	P	0.000044
AFFX-DapX-5_at	2.2	A	0.544587
AFFX-DapX-M_at	7.7	A	0.368438
AFFX-DapX-3_at	1.5	A	0.960339
AFFX-LysX-5_at	1.2	A	0.883887
AFFX-LysX-M_at	14.1	A	0.699394
AFFX-LysX-3_at	3.1	A	0.631562
AFFX-PheX-5_at	1.5	A	0.904333
AFFX-PheX-M_at	1.9	A	0.852061
AFFX-PheX-3_at	4.3	A	0.852061
AFFX-ThrX-5_at	2	A	0.921998
AFFX-ThrX-M_at	11.7	A	0.287743

Total number of rows: 22690

Table truncated, full table size 610 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM150266.CEL.gz	3.3 Mb	(ftp)(http)	CEL