|
| Status |
Public on Aug 03, 2015 |
| Title |
7day_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
7 day differentiated Adipose Derived Stem Cells
|
| Organism |
Homo sapiens |
| Characteristics |
days differentiated: 7 day
gender: female
tissue: abdominal subcutaneous adipose
cell type: differentiated Adipose Derived Stem Cells
|
| Treatment protocol |
Cells cultured in ASC plating media served as undifferentiated Day 0 controls. The differentiation media was replaced every other day for either 7 days or 21 days.
|
| Growth protocol |
Adipose-derived stem cells (ASCs) were isolated from abdominal subcutaneous adipose tissue. Cells cultured in ASC plating media served as undifferentiated Day 0 controls or grown for 7 or 21 days in Adiposite differentiation media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from undifferentiated ASCs, and cells subjected to 7 and 21-day differentiated protocols was extracted using the RNeasy Mini Kit (Qiagen Inc., Valencia, CA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
In vitro transcription was performed using the Ambion Message Amp Premier Enhanced assay protocol (Ambion Inc, Austin, TX) starting with 500 ng of purified total RNA.
|
| |
|
| Hybridization protocol |
Fifteen micrograms of purified, amplified, biotin labeled cRNA was fragmented and hybridized onto Affymetrix Human Genome HGU133A plus 2.0 arrays following manufacturer's recommendations
|
| Scan protocol |
Washing, staining and scanning of arrays was performed on the Affymetrix Fluidics Station 450 and Scanner 3000 immediately after completion of hybridization.
|
| Data processing |
Microarray data was processed using the Expression Console (Affymetrix) with signal intensity calculated by Microarray Suite version 5.0 (MAS 5.0).
|
| |
|
| Submission date |
Sep 10, 2014 |
| Last update date |
Aug 03, 2015 |
| Contact name |
Sandeep Kathju |
| Organization name |
University of Pittsburgh
|
| Department |
Plastic Surgery
|
| Lab |
E1607 BST
|
| Street address |
200 Lothrop Str
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE61302 |
Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage |
|
