|
| Status |
Public on Sep 04, 2014 |
| Title |
anti-IgM KO |
| Sample type |
RNA |
| |
|
| Source name |
anti-IgM KO
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6/C57
cell type: splenic B cells
agent: anti-IgM
|
| Treatment protocol |
B cells were either cultured in media, or activated with anti-IgM, anti-CD40 or LPS for 4 hours
|
| Growth protocol |
primary B cells were isolated from mouse spleens and cultured in RPMI supplemented with 10% FBS and 1% pen/strep for 4 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Qiagen Rneasy mini kit, and DNAse I treated per manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
biotin labeled cRNA performed by University of Chicago Genomics core facility
|
| |
|
| Hybridization protocol |
standard Illumina hybridization protocol performed by University of Chicago Genomics core facility
|
| Scan protocol |
standard Illumina scanning protocol performed by University of Chicago Genomics core facility
|
| Description |
RNA pooled from 3 mice
DF 4
|
| Data processing |
data was background substracted quantile normalized with Genomestudio performed by University of Chicago genomics core facility
|
| |
|
| Submission date |
Sep 03, 2014 |
| Last update date |
Sep 04, 2014 |
| Contact name |
Sin Yi Kong |
| E-mail(s) |
[email protected]
|
| Phone |
312-503-3023
|
| Organization name |
Northwestern University
|
| Department |
pathology
|
| Lab |
Deyu Fang
|
| Street address |
300 E Chicago
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60601 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE61029 |
Gene expression analysis of DBC1 KO B cells |
|
