|
Status |
Public on Aug 28, 2014 |
Title |
male saline non-enriched ventral striatum replicate 3 |
Sample type |
RNA |
|
|
Source name |
male saline non-enriched ventral striatum
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Long Evans gender: male age: postnatal day 42 brain region: Ventral striatum prenatal exposure: Saline postnatal social treatment: Non-enriched
|
Treatment protocol |
N/A
|
Growth protocol |
N/A
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen total RNA
|
Label |
biotin
|
Label protocol |
100 ng of total RNA was prepared for hybridization using the WT Expression Kit (Ambion) according to manufacturer protocol
|
|
|
Hybridization protocol |
Affymetrix EukGE-WS2v4 Protocol
|
Scan protocol |
Standard Affymetrix Genechip 7G Scanner
|
Description |
mcnv_35
|
Data processing |
Partek Genomics Suite 6.5 to perform RMA normalization; the data were filtered afterwards to include only rat transcripts since the experiments were done with rat RNA.
|
|
|
Submission date |
Aug 27, 2014 |
Last update date |
Aug 28, 2014 |
Contact name |
Frank A Middleton |
E-mail(s) |
[email protected]
|
Phone |
315-464-7721
|
Organization name |
SUNY Upstate Medical Univ
|
Department |
Neuroscience and Physiology
|
Street address |
505 Irving Avenue
|
City |
Syracuse |
State/province |
NY |
ZIP/Postal code |
13210 |
Country |
USA |
|
|
Platform ID |
GPL6247 |
Series (2) |
GSE60819 |
Effects of acute prenatal exposure to ethanol on microRNA expression are ameliorated by environmental manipulation. |
GSE60901 |
Comparison of small RNA-seq and microarray analysis for determining the effects of acute prenatal ethanol exposure on microRNA expression and its amelioration by environmental manipulation |
|