|
Status |
Public on Feb 02, 2015 |
Title |
CTR_KA_rep.2 |
Sample type |
RNA |
|
|
Source name |
denate gyrus of hippocampus
|
Organism |
Mus musculus |
Characteristics |
time: 6 hrs drug: Kainic acid genotype: CTR (SRF f/f)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit followed by clean-up and DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Data processing |
The data were preprocessed using beadarray R package and normalized using quantile normalization.
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|
|
Submission date |
Aug 26, 2014 |
Last update date |
Feb 02, 2015 |
Contact name |
Marcin Piechota |
E-mail(s) |
[email protected]
|
Phone |
(+4812) 6623328
|
Organization name |
Institute of Pharmacology PAS
|
Department |
Molecular Neuropharmacology
|
Street address |
Smetna 12
|
City |
Krakow |
ZIP/Postal code |
31-343 |
Country |
Poland |
|
|
Platform ID |
GPL17543 |
Series (1) |
GSE60772 |
Genome-wide analysis of kainic acid-induced gene expression in dentate gyrus from CTR and SRF knockout mice. |
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