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Sample GSM148684 Query DataSets for GSM148684
Status Public on Dec 13, 2006
Title Untreated Control 12weeks AffyGC RMA rep8
Sample type RNA
 
Source name Right brain hemisphere ,C57BL male mouse,Un-treated Control,12 weeks
Organism Mus musculus
Characteristics Tissue: Forebrain (left hemisphere), Strain: C57BL, Gender: Male, Age: 4weeks, Drug treatment: Untreated-Control, Period of treatment: 12 weeks, Method of sacrificing: cervical dislocation.
Treatment protocol Mouse was housed individually in a separate cage under a 12-h light–dark cycle and permitted food and water ad libitum. A period of acclimatization for two weeks was allowed prior to any experimental procedure. No treatment was added to the animal’s drinking water .The weight and water consumption was measured weekly. At the end of 12 weeks animal was sacrificed by cervical dislocation and the brain was removed, dissected into left and right hemispheres and cerebellum, flash frozen in liquid nitrogen and stored at -80 C for future use.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8-10 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene expression data from the right forebrain of C57BL mouse received no treatment for twelve weeks.
Data processing Normalized expression values were calculated from probe level data (CEL file) using the function of Robust Multi-Array expression measure using sequence information GC-RMA (Wu et al 2004, www.bepress.com/jhubiostat/paper4).Computational steps were implemented in the R-language. GCRMA and it's associated packages were provided by the open source bioconductor project.
 
Submission date Dec 06, 2006
Last update date Dec 12, 2006
Contact name Mie A Rizig
E-mail(s) [email protected], [email protected]
Phone +44 (0)2076799436
Fax +44 (0)2076799437
Organization name University Collage London
Department Mental Health Sciences
Lab Molecular Psychiatry Lab
Street address Windeyer Institute of Medical Sciences,46 Cleveland Street
City London
ZIP/Postal code W1T 4JF
Country United Kingdom
 
Platform ID GPL339
Series (2)
GSE6467 Twelve weeks expression data of the antipsychotics Clozapine and Haloperidol in the mouse brain (Affymetrix, GCRMA).
GSE6512 Clozapine and Haloperidol effect on the mouse brain

Data table header descriptions
ID_REF Probe ID, Affymetrix GeneChip Mouse Expression Array 430A
VALUE Normalized intensity value calculated from Probe level data (CEL file) by means of GC-RMA.

Data table
ID_REF VALUE
1415670_at 10.0147358
1415671_at 11.10195347
1415672_at 9.921257166
1415673_at 7.666124407
1415674_a_at 8.030573566
1415675_at 7.915782617
1415676_a_at 10.4499153
1415677_at 8.686293613
1415678_at 9.269351788
1415679_at 9.522472912
1415680_at 7.162753856
1415681_at 7.543543392
1415682_at 8.583859278
1415683_at 9.204314819
1415684_at 6.37846791
1415685_at 7.130956266
1415686_at 9.593133584
1415687_a_at 12.6911382
1415688_at 9.019376122
1415689_s_at 7.111983898

Total number of rows: 22690

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM148684.cel.gz 2.2 Mb (ftp)(http) CEL

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