|
| Status |
Public on Dec 13, 2006 |
| Title |
Clozapine treated 12weeks AffyGC RMA rep7 |
| Sample type |
RNA |
| |
|
| Source name |
Right brain hemisphere ,C57BL male mouse,Clozapine treated,Orally,12 weeks
|
| Organism |
Mus musculus |
| Characteristics |
Tissue: Forebrain (left hemisphere), Strain: C57BL, Gender: Male, Age: 4weeks, Drug treatment: Atypical antipsychotic Clozapine, Dose: 12mg/kg/day, Route of treatment: Oral treatment-Drug added to drinking water, Period of treatment: 12 weeks, Method of sacrificing: cervical dislocation.
|
| Treatment protocol |
Mouse was housed individually in a separate cage under a 12-h light–dark cycle and permitted food and water ad libitum. A period of acclimatization for two weeks was allowed prior to any experimental procedure. Clozapine was added to the animal’s drinking water in a concentration equivalent to the highest human clinical dosage (i.e. 12mg/kg/day for Clozapine) and delivered through light protected bottles. The weight and water consumption was measured weekly and the drug concentration in water was adjusted accordingly .At the end of 12 weeks animal was sacrificed by cervical dislocation and the brain was removed, dissected into left and right hemispheres and cerebellum, flash frozen in liquid nitrogen and stored at -80 C for future use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8-10 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from the right forebrain of C57BL mouse received Clozapine oral treatment for twelve weeks..
|
| Data processing |
Normalized expression values were calculated from probe level data (CEL file) using the function of Robust Multi-Array expression measure using sequence information GC-RMA (Wu et al 2004, www.bepress.com/jhubiostat/paper4).Computational steps were implemented in the R-language. GCRMA and it's associated packages were provided by the open source bioconductor project.
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| |
|
| Submission date |
Dec 06, 2006 |
| Last update date |
Dec 12, 2006 |
| Contact name |
Mie A Rizig |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
+44 (0)2076799436
|
| Fax |
+44 (0)2076799437
|
| Organization name |
University Collage London
|
| Department |
Mental Health Sciences
|
| Lab |
Molecular Psychiatry Lab
|
| Street address |
Windeyer Institute of Medical Sciences,46 Cleveland Street
|
| City |
London |
| ZIP/Postal code |
W1T 4JF |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL339 |
| Series (2) |
| GSE6467 |
Twelve weeks expression data of the antipsychotics Clozapine and Haloperidol in the mouse brain (Affymetrix, GCRMA). |
| GSE6512 |
Clozapine and Haloperidol effect on the mouse brain |
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