|
| Status |
Public on Nov 04, 2014 |
| Title |
midbrain_Pink1 -/- || A53T-SNCA_6 months_replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
midbrain,Pink1 -/- || A53T-SNCA,6 months
|
| Organism |
Mus musculus |
| Characteristics |
tissue: midbrain
genotype/variation: Pink1 -/- || A53T-SNCA
age: 6 months
|
| Treatment protocol |
not applicable
|
| Growth protocol |
Mice were housed in Type II L cages (365x207x140 mm, floor area 530 cm2; IVC-based)at an environmental temperature 20-24°C, humidity: 55±10%and under 12/12 h light/dark cycle with food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the 3'IVT Express Kit (Ambion) from 100ng total RNA according to the manufacturer.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HT MG-430 PM Array Plates. Plates were hybridized, washed and stained in the Affymetrix Gene Titan.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Titan instrument with AGCC 3.0 software.
|
| Data processing |
The data were analysed using Bioconductor package 'affy' (1.28.0) under R-2.12.0. Background correction, normalization and probe summarization was performed with RMA.
|
| |
|
| Submission date |
Aug 13, 2014 |
| Last update date |
Nov 04, 2014 |
| Contact name |
Michael H. Walter |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Tuebingen
|
| Department |
Department of Medical Genetics
|
| Lab |
The Microarray Facility Tübingen
|
| Street address |
Calwer Str. 7
|
| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE60414 |
Potentiation of neurotoxicity in double mutant mice with Pink1 ablation and A53T-SNCA overexpression |
|
