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Status
Public on Dec 01, 2014
Title
C16
Sample type
RNA
Source name
control
Organism
Homo sapiens Characteristics
cell type: H tert Fibroblasts
Treatment protocol
HTert-immortalized fibroblasts from C1, C6, C16, P1, P2 and P3 were treated with 1000U of IFN-α2b for 12 h, washed with PBS and left to rest for 36 h, after which relative mRNA levels were assessed
Extracted molecule
total RNA
Extraction protocol
RNAeasy kits from Qiagen
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA.
Hybridization protocol
Standard Affymetrix protocol was perfomed
Scan protocol
GeneChips were scanned using standard GeneArray Scanner.
Description
C16
Data processing
Affymetrix Console and TAC
Submission date
Aug 12, 2014
Last update date
Dec 01, 2014
Contact name
Dusan Bogunovic
E-mail(s)
[email protected] Organization name
Icahn School of Medicine at Mount Siani
Department
Microbiology
Street address
1468 Madison Avenue,
City
New York
State/province
New York
ZIP/Postal code
10029
Country
USA
Platform ID
GPL17586 Series (1)
GSE60359 ISG15 in regulation of interferon responses
Data table header descriptions ID_REF VALUE log2 rma-gene-full
Data table ID_REF VALUE TC01000001.hg.1
6.69323
TC01000002.hg.1
5.67964
TC01000003.hg.1
3.23715
TC01000004.hg.1
2.93078
TC01000005.hg.1
7.48185
TC01000006.hg.1
11.5108
TC01000007.hg.1
11.4413
TC01000008.hg.1
5.526
TC01000009.hg.1
2.61962
TC01000010.hg.1
4.2647
TC01000011.hg.1
6.70517
TC01000012.hg.1
4.21832
TC01000013.hg.1
8.37297
TC01000014.hg.1
2.79513
TC01000015.hg.1
5.56593
TC01000016.hg.1
4.07524
TC01000017.hg.1
6.34561
TC01000018.hg.1
8.02871
TC01000019.hg.1
6.66278
TC01000020.hg.1
7.26129
70523 1655 Kbytes .Supplementary file Size Download File type/resource GSM1474241_7_HTA_2.0_Dusan_08132013_HTA-2_0_.CEL.gz
25.3 Mb
(ftp) (http) CEL
GSM1474241_7_HTA_2.0_Dusan_08132013_HTA-2_0_.RMA.rma-gene-full.chp.gz
457.6 Kb
(ftp) (http) CHP
Processed data included within Sample table Processed data provided as supplementary file
