Colons were dissected, epithelial cells were liberated by treatment with 5mM EDTA
Growth protocol
mice in gnotobiotic isolators
Extracted molecule
total RNA
Extraction protocol
Qiagen Rneasy Mini
Label
biotin
Label protocol
Illumina TotalPrep RNA
Hybridization protocol
Standard Illumina Protocol
Scan protocol
Illumina BeadStudio
Description
gnotobiotic animals
Data processing
Background subtraction and quantile normalization was performed with Illumina software Raw or Background-subtracted, quantile normalized intensities from Illumina BeadStudio
