|
| Status |
Public on Nov 18, 2006 |
| Title |
larrydissectmes5 |
| Sample type |
RNA |
| |
|
| Source name |
metanephric mesenchyme
|
| Organism |
Mus musculus |
| Characteristics |
gene reported: wild-type
strain: CD-1
sex: unknown
developmental stage: E11.5
theiler stage: 19
somite count: NA
developmental landmark: NA
|
| Treatment protocol |
pool size: Approx 50 sections from 5 kidneys
Pooled sample: Yes
Dissection Method: Laser capture microdissection
CD-1 mice are time-mated. Pregnant CD-1 mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal kidneys are dissected from fetuses placed in OCT in a mold, frozen in liquid nitrogen and stored at -80°C. Blocks are sectioned, stained and used for laser capture micro-dissection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Potter protocol: 'RNA purification'
|
| Label |
Biotin
|
| Label protocol |
Biotin-X-X-NHS (EPICENTRE Biotechnologies)
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
Amount labeled target hybridization to array: 5ug
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter protocols
Rounds of amplification: 2
URL: http://gudmap.hgu.mrc.ac.uk/gudmap/pages/mic_submission.html?id=GUDMAP:7108
|
| Data processing |
Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
|
| |
|
| Submission date |
Nov 15, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
[email protected]
|
| Phone |
+44 131 651 8500
|
| Organization name |
IGMM MRC Human Genetics Unit
|
| Lab |
GUDMAP Database Group
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6290 |
Gene expression profiles of components isolated from developing kidney at E11.5, E12.5 & E15.5 using LCM. (GUDMAP Series ID: 10) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
