|
| Status |
Public on Mar 13, 2015 |
| Title |
Match normal of fusion positive tumor; biological rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Human salivary gland tumor patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Adenoid cystic tumor
sample type: Match normal of fusion positive tumor
|
| Treatment protocol |
Samples were excised from human patients under approved IRB protocols
|
| Growth protocol |
Samples were collected from fresh frozen tissues
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
200ng total RNA was labeledusing Ambion WT expression Kit following manufacturer's instructions. cDNA was fragmented and labeled using the Affymetrix GeneChip WT terminal labeling kit according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of cDNA were hybridized for 16 hr at 45C on Affymetrix Human Gene ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
The GeneChip arrays were stained and scanned in an Affymetrix GCS 3000 7G Scanner.
|
| Description |
gene expression array for normal tissue
|
| Data processing |
The data were analyzed with Affymetrix Expression Console using RMA algorithm followed by log2 transformation.
|
| |
|
| Submission date |
Jul 23, 2014 |
| Last update date |
Mar 13, 2015 |
| Contact name |
Nicholas Navin |
| Organization name |
UT MD Anderson
|
| Street address |
6767Bertner AVE
|
| City |
Houston |
| State/province |
tx |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE59701 |
A unifying gene signature for adenoid cystic cancer identifies parallel MYB-dependent and MYB-independent therapeutic targets (mRNA) |
| GSE59702 |
A unifying gene signature for adenoid cystic cancer identifies parallel MYB-dependent and MYB-independent therapeutic targets |
|
