|
| Status |
Public on Jun 01, 2017 |
| Title |
WAT - before bariatric surgery - rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Abdominal subcutaneous WAT
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Abdominal subcutaneous white adipose (WAT)
gender: Female
obesity status: before bariatric surgery
|
| Treatment protocol |
Tissues were obtained by needle aspiration under local anasthesia
|
| Growth protocol |
Human abdominal subcutaneous white adipose tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen, Hilden, Germany) according to the instructions provided by the manufacturer
|
| Label |
Biotin
|
| Label protocol |
Total RNA was amplified and biotin labeled using the Whole Transcript (WT) Sense Target Labeling Protocol
|
| |
|
| Hybridization protocol |
Biotinylated RNA was hybridized to Gene 1.1 ST Arrays, and then washed, stained and scanned using standardized protocols (Affymetrix Inc., Santa Clara, CA).
|
| Scan protocol |
Standardized Affymetrix protocols (Affymetrix Inc., Santa Clara, CA)
|
| Description |
Gene expression profiling based on human abdominal subcutaneous white adipose tissue obtained by needle aspiration
|
| Data processing |
Results were processed using the RMA method
|
| |
|
| Submission date |
Jul 02, 2014 |
| Last update date |
Jun 01, 2017 |
| Contact name |
Niklas Mejhert |
| Organization name |
Karolinska Institutet
|
| Street address |
Hälsovägen 7
|
| City |
Stockholm |
| ZIP/Postal code |
141 86 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE59034 |
A Memory for Obesity in Adipose Tissue |
|
