|
| Status |
Public on May 18, 2015 |
| Title |
eWAT_HFD_WT_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
WT_HFD 16wks_epidydimal white adipose
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
genotype/variation: wild type
diet: high fat diet (HFD) for 16 wks
tissue: epidydimal white adipose tissue
|
| Treatment protocol |
After a 2 week run-in period on a low fat diet (LFD), male mice were given a high-fat diet (HFD) diet for 16 weeks. The LFD and HFD contained 10% respecytively 45% of energy derived from fat (diet formulations according to D12450B resp. D12451; Research Diets, Inc). After the 16 week diet intervention, epididymal white adipose tissue (eWAT) was removed, and stored at -800 until analysis.
|
| Growth protocol |
The IL-37tg mice on a C57Bl/6 background have been reported before (Nold et al, Nat Immunol, 2010; PMID: 20935647). Wild type C57BL/6 mice were purchased from the Jackson Laboratories. All animal procedures were conducted under protocols approved by the animal experimentation committee of Radboud University Medical Centre, Nijmegen, the Netherlands.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy micro kit from Qiagen. RNA integrity was checked on chip analysis (Agilent 2100 bioanalyzer, Agilent Technologies, Amsterdam, the Netherlands) according to the manufacturer's instructions. RNA was judged as suitable for array hybridization only if samples exhibited intact bands corresponding to the 18S and 28S ribosomal RNA subunits, and displayed no chromosomal peaks or RNA degradation products (RNA Integrity Number > 8.0).
|
| Label |
biotin
|
| Label protocol |
One hundred nanogram of RNA was used for whole transcript cDNA synthesis with the Ambion WT expression kit [catalog number 4411974] (Applied Biosystems/Life Technologies, Nieuwekerk a/d IJssel, The Netherlands).
|
| |
|
| Hybridization protocol |
Hybridization and washing of the Affymetrix GeneChip Mouse Gene 1.1 ST peg arrays were performed on a GeneTitan Instrument (Affymetrix, Santa Clara, CA) according to the manufacturer's recommendations.
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneTitan instrument (Affymetrix, Santa Clara, CA).
|
| Description |
G083_F09_02_WT_HFD.CEL
|
| Data processing |
Expression estimates were calculated applying the RMA algorithm in the Bioconductor library 'Oligo' (v1.28.2).
|
| |
|
| Submission date |
Jun 30, 2014 |
| Last update date |
May 18, 2015 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
[email protected]
|
| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
|
| Lab |
Nutrition, Metabolism & Genomics Group
|
| Street address |
HELIX, Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE58952 |
IL-37 protects against obesity-induced inflammation and insulin resistance |
|
