|
| Status |
Public on Jul 01, 2014 |
| Title |
Staphylococcus aureus planktonic cells_Licochalcone A 60 min_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
Staphylococcus aureus planktonic cells_Licochalcone A
|
| Organism |
Staphylococcus aureus |
| Characteristics |
strain: Standard strain ATCC29213
cell type: planktonic cells
|
| Treatment protocol |
Licochalcone A stock solution, prepared in dimethyl sulfoxide (DMSO), was added to the cultures, giving a final concentration of 1/2× MIC (2 μg/ml), and the final concentration of DMSO was 0.1% (vol/vol) as control group. Biofilms washed once with PBS to remove any unbound bacteria, Licochalcone A stock solution, prepared in dimethyl sulfoxide (DMSO), was added to the cultures, giving a final concentration of 4× MIBC (64 μg/ml), and the final concentration of DMSO was 0.1% (vol/vol) as control group. Then, the bacterial planktonic and biofilm cells were further incubated for 60 min at 37°C
|
| Growth protocol |
S. aureus strain ATCC29213 was grown overnight at 200 rpm in a rotary shaker at 37°C in 10 ml of TSB, after 12 hours, the culture were inoculated to an initial OD600 of 0.05 in 100 ml of TSB in 250-ml Erlenmeyer flasks, the planktonic cells were grown at 37°C at 200 rpm to an OD600 of 0.3. Within biofilm, the overnight culture were inoculated to an initial OD600 and diluted in TSBg to obtain the final concentration of 1× 105cfu/ml, 30 ml bacterial suspension diluted in 2% glucose-supplemented TSB containing 1 × 105 cfu/ml into the culture dish, then incubated in air for 48 h at 37°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total bacterial RNA was isolated as described by Hutter et al., 2004, 48(8):2838-2844
|
| Label |
biotin
|
| Label protocol |
Conversion to cDNA and labeling was performed as described in Hutter et al., Antimicrobial agents and chemotherapy, 2004, 48(8):2838-284
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
Gene expression data from Staphylococcus aureus planktonic cells with Licochalcone A treatment at 60min
|
| Data processing |
Affymetrix GeneChip Opererating Software (GCOS) version 1.0
|
| |
|
| Submission date |
Jun 30, 2014 |
| Last update date |
Jul 01, 2014 |
| Contact name |
lu yu |
| E-mail(s) |
[email protected]
|
| Phone |
+8643187836713
|
| Organization name |
Jilin University
|
| Department |
Institute of Zoonosis
|
| Lab |
Key Laboratory of Zoonosis Reaserch
|
| Street address |
Xi’an Road 5333#
|
| City |
Changchun |
| State/province |
Jilin |
| ZIP/Postal code |
130062 |
| Country |
China |
| |
|
| Platform ID |
GPL1339 |
| Series (1) |
| GSE58938 |
Phenotype and expression profile analysis of Staphylococcus aureus biofilms and planktonic cells in response to Licochalcone A |
|
