|
| Status |
Public on Jan 30, 2015 |
| Title |
Breast cancer_cell line_untreated_DNA_MDAMB453 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
HER2 positive breast cancer cell line, untreated
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
her2 status: positive
|
| Treatment protocol |
No treatment
|
| Growth protocol |
All cells were grown according to protocol provided by manufacturer.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
TriZOL
|
| Label |
Cy5
|
| Label protocol |
One μg of digested and purified tumor and reference DNA was labeled with Cy5-dUTP and Cy3-dUTP (Perkin–Elmer, Wellesley, MA), respectively, in a random priming reaction using BioPrime DNA Labeling System (Invitrogen, Carlsbad, CA).
|
| |
|
| Channel 2 |
| Source name |
Normal female DNA_reference
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
|
| Treatment protocol |
No treatment
|
| Growth protocol |
All cells were grown according to protocol provided by manufacturer.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
TriZOL
|
| Label |
Cy3
|
| Label protocol |
One μg of digested and purified tumor and reference DNA was labeled with Cy5-dUTP and Cy3-dUTP (Perkin–Elmer, Wellesley, MA), respectively, in a random priming reaction using BioPrime DNA Labeling System (Invitrogen, Carlsbad, CA).
|
| |
|
| |
| Hybridization protocol |
Labeled tumor and reference samples were pooled and hybridized onto the arrays according to the protocol provided by Agilent Technologies.
|
| Scan protocol |
The arrays were scanned with a laser scanner (G2565 Scanner, Agilent Technologies).
|
| Data processing |
Feature Extraction software version 7.5.1 (Agilent Technologies) was used for the pre-processing of the signal intensities to obtain normalized intensity ratios.
|
| |
|
| Submission date |
Jun 27, 2014 |
| Last update date |
Jan 30, 2015 |
| Contact name |
Sandra Jernström |
| E-mail(s) |
[email protected]
|
| Organization name |
OUS The Norwegian Radium hospital
|
| Department |
Genetics
|
| Street address |
Ullernchausséen 70
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL9128 |
| Series (2) |
| GSE58886 |
Drug screening and genomic analyses of HER2 positive breast cancer cell lines reveal predictors for treatment response [CGH] |
| GSE58887 |
Drug screening and genomic analyses of HER2 positive breast cancer cell lines reveal predictors for treatment response |
|
