Rat hearts were dissected out, briefly profused, right ventricle was dissected, snapfrozen in Liquid nitrogen. Frozen tissue was powdered in liquid nitrogen and tissue powder used for RNA extraction.
Extracted molecule
total RNA
Extraction protocol
Trizol RNA extraction followed by Qiagen Clean up.
Label
biotin
Label protocol
Sample was labelled by Affymetrix standard protocol
Hybridization protocol
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on RAT 230-2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol
GeneChips were scanned using the Agilent Gene Array Scanner G2500A
Description
Gene expression data from Rat right ventricle, 18 hour high dose microsphere bead induced PE sample.
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
