|
Status |
Public on Apr 01, 2008 |
Title |
control participant P313 |
Sample type |
RNA |
|
|
Source name |
human CD14 positive monopcytes
|
Organism |
Homo sapiens |
Characteristics |
cell type: monocyte disease state: control participant
|
Treatment protocol |
monocytes were precipitated untouched from 8ml peripheral blood mononuclear cells, washed in PBS + 2mM EDTA, lysed in TriZol
|
Extracted molecule |
total RNA |
Extraction protocol |
the RNA was worked up using PLG Tubes and the Qiagen Rneasy Kit
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133A2.0 GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
Description |
Gene expression data from control participant SH
|
Data processing |
The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method.
|
|
|
Submission date |
Oct 16, 2006 |
Last update date |
Aug 28, 2018 |
Contact name |
Sandy Mosig |
E-mail(s) |
[email protected], [email protected]
|
Phone |
+49-3641-934813
|
Fax |
+49-3641-933950
|
Organization name |
University Hospital Jena
|
Department |
Molecular Hemostaseology - Institute of Vascular Medicine
|
Street address |
Bachstrasse 18
|
City |
Jena |
ZIP/Postal code |
07743 |
Country |
Germany |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE6054 |
Monocytes of patients with familial hypercholesterolemia show alterations in cholesterol metabolism |
|
Relations |
Reanalyzed by |
GSE49910 |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE119087 |