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Sample GSM140245 Query DataSets for GSM140245
Status Public on Apr 01, 2008
Title control participant P307
Sample type RNA
 
Source name human CD14 positive monopcytes
Organism Homo sapiens
Characteristics cell type: monocyte
disease state: control participant
Treatment protocol monocytes were precipitated untouched from 8ml peripheral blood mononuclear cells, washed in PBS + 2mM EDTA, lysed in TriZol
Extracted molecule total RNA
Extraction protocol the RNA was worked up using PLG Tubes and the Qiagen Rneasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133A2.0 GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from control participant KP
Data processing The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Oct 16, 2006
Last update date Aug 28, 2018
Contact name Sandy Mosig
E-mail(s) [email protected], [email protected]
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
 
Platform ID GPL570
Series (1)
GSE6054 Monocytes of patients with familial hypercholesterolemia show alterations in cholesterol metabolism
Relations
Reanalyzed by GSE49910
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF Affymetrix annotation number
VALUE GCOS 1.4 calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1128.4 P 0.000446
AFFX-BioB-M_at 2633.9 P 0.000044
AFFX-BioB-3_at 1186.3 P 0.000044
AFFX-BioC-5_at 4839.2 P 0.000044
AFFX-BioC-3_at 6182.5 P 0.000044
AFFX-BioDn-5_at 9581.4 P 0.000044
AFFX-BioDn-3_at 20643.3 P 0.000044
AFFX-CreX-5_at 46254.4 P 0.000052
AFFX-CreX-3_at 61038.9 P 0.000044
AFFX-DapX-5_at 1396.4 P 0.000146
AFFX-DapX-M_at 2295.3 P 0.000662
AFFX-DapX-3_at 2824.7 P 0.000052
AFFX-LysX-5_at 185 P 0.000662
AFFX-LysX-M_at 295.3 P 0.033677
AFFX-LysX-3_at 549.9 P 0.000044
AFFX-PheX-5_at 235.5 P 0.000127
AFFX-PheX-M_at 396.6 P 0.002023
AFFX-PheX-3_at 525.8 P 0.00039
AFFX-ThrX-5_at 316.6 P 0.002556
AFFX-ThrX-M_at 529 P 0.000095

Total number of rows: 54675

Table truncated, full table size 1445 Kbytes.




Supplementary file Size Download File type/resource
GSM140245.CEL.gz 7.9 Mb (ftp)(http) CEL

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