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Sample GSM140239 Query DataSets for GSM140239
Status Public on Apr 01, 2008
Title heterozygot FH P279
Sample type RNA
 
Source name human CD14 positive monopcytes
Organism Homo sapiens
Characteristics cell type: monocyte
disease state: heterozygote FH
Treatment protocol monocytes were precipitated untouched from 8ml peripheral blood mononuclear cells, washed in PBS + 2mM EDTA, lysed in TriZol
Extracted molecule total RNA
Extraction protocol the RNA was worked up using PLG Tubes and the Qiagen Rneasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133A2.0 GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from the heterozygous FH participant F
Data processing The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Oct 16, 2006
Last update date Aug 28, 2018
Contact name Sandy Mosig
E-mail(s) [email protected], [email protected]
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
 
Platform ID GPL570
Series (1)
GSE6054 Monocytes of patients with familial hypercholesterolemia show alterations in cholesterol metabolism
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF Affymetrix annotation number
VALUE GCOS 1.4 calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 871.6 P 0.000509
AFFX-BioB-M_at 1304.5 P 0.000044
AFFX-BioB-3_at 855.2 P 0.000169
AFFX-BioC-5_at 2988.9 P 0.000052
AFFX-BioC-3_at 3833.2 P 0.000052
AFFX-BioDn-5_at 6537.4 P 0.000044
AFFX-BioDn-3_at 16466.7 P 0.000052
AFFX-CreX-5_at 35094.8 P 0.000052
AFFX-CreX-3_at 41557.8 P 0.000044
AFFX-DapX-5_at 634.6 P 0.000044
AFFX-DapX-M_at 1583.2 P 0.000754
AFFX-DapX-3_at 2479 P 0.00006
AFFX-LysX-5_at 120.8 P 0.006187
AFFX-LysX-M_at 189.4 A 0.147939
AFFX-LysX-3_at 394.9 P 0.00006
AFFX-PheX-5_at 176.5 P 0.000225
AFFX-PheX-M_at 253.2 P 0.002867
AFFX-PheX-3_at 460.9 P 0.000857
AFFX-ThrX-5_at 216.5 P 0.026111
AFFX-ThrX-M_at 370.5 P 0.001102

Total number of rows: 54675

Table truncated, full table size 1444 Kbytes.




Supplementary file Size Download File type/resource
GSM140239.CEL.gz 7.7 Mb (ftp)(http) CEL

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