|
| Status |
Public on Jan 01, 2007 |
| Title |
HE140 CMara 75min 3 |
| Sample type |
RNA |
| |
|
| Source name |
Ustilago maydis
|
| Organism |
Mycosarcoma maydis |
| Characteristics |
Strain HE140 is a derivative of FB1 carrying the adr1 gene under the control of the arabinose inducible crg1 promotor in single copy in the Cbx locus of U. maydis.
|
| Biomaterial provider |
Heiko Eichhorn and Regine Kahmann
|
| Treatment protocol |
none
|
| Growth protocol |
HE140 was grown to a OD600 of 0.5 at 28°C in CM medium (Holliday, 1974) containing 1 % (w/v) glucose. Subsequently, cells were shifted to CM medium containing 1 % (w/v) arabinose instead of glucose as a carbon source and incubated for 75 min.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the TRIZOL-reagent (Invitrogen) according to the manufacturer´s instructions. RNA samples were further column purified (Qiagen RNeasy) and the quality checked using a Bioanalyser with a RNA6000 Nano LabChip Kit (Agilent).
|
| Label |
biotin/ phycoerythrin
|
| Label protocol |
Target preparation was performed according to the standard Affymetrix protocol using the Enzo BioArray RNA transcript labeling kit, except a reaction temperature of 50°C for first strand cDNA synthesis.
|
| |
|
| Hybridization protocol |
Affymetrix EukEG-WS2 on a GeneChip Fluidics Station 400
|
| Scan protocol |
Affymetrix GCS2500
|
| Description |
Experiment to measure the efects of Adr1 induction in U. maydis. Free Adr1 signals a high level of cAMP.
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Oct 14, 2006 |
| Last update date |
Oct 27, 2006 |
| Contact name |
Britta Winterberg |
| E-mail(s) |
[email protected]
|
| Phone |
06421 178650
|
| Fax |
06421 178599
|
| Organization name |
Max-Planck-Institut für terrestrische Mikrobiologie
|
| Department |
Orgnaismische Interaktionen
|
| Lab |
Schirawski
|
| Street address |
Karl-von-Frisch-Str.
|
| City |
Marburg |
| ZIP/Postal code |
D-35043 |
| Country |
Germany |
| |
|
| Platform ID |
GPL3681 |
| Series (1) |
| GSE6039 |
Identification of Adr1 regulated genes |
|
