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Status
Public on Feb 01, 2007
Title
UCB-MSC_UCB2_rep1B
Sample type
RNA
Source name
human umbilical cord blood-derived mesenchymal stem cell population 2, replicate 1
Organism
Homo sapiens
Characteristics
Tissue: umbilical cord blood-derived mesenchymal stem/stromal cell
Biomaterial provider
Dr Biagio Saitta, Coriell Institute for Medical Research, 403 Haddon Ave, Camden, NJ 08103, USA, [email protected] http://www.coriell.org/ Coriell
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from cells in culture using the RNAeasy Mini Kit (Qiagen), according to manufacturer's protocol
Label
Biotin
Label protocol
Microarray target was prepared following the Affymetrix protocol. In brief, cDNA was synthesized from total RNA using the SuperScript II double-stranded cDNA synthesis kit (Invitrogen), followed by in vitro transcription, where labeled cRNA was generated (Enzo).
Description
none
Data processing
RMA method implemented in BioConductor
Submission date
Oct 12, 2006
Last update date
Oct 13, 2006
Contact name
Kenro Kusumi
E-mail(s)
[email protected]
Phone
480-727-8993
Fax
480-965-6899
URL
http://sols.asu.edu/faculty/kkusumi.php
Organization name
Arizona State University
Department
School of Life Sciences
Street address
PO Box 874501
City
Tempe
State/province
AZ
ZIP/Postal code
85287
Country
USA
Platform ID
GPL97
Series (1)
GSE6029
Cord Blood-Derived Mesenchymal Stem Cells with Distinct Growth Kinetics, Differentiation Potentials, Expression Profiles
Data table header descriptions
ID_REF
VALUE
RMA expression estimate
Data table
ID_REF
VALUE
200000_s_at
8.41100376142294
200001_at
10.5259080675725
200002_at
10.9959817039438
200003_s_at
11.32621767106
200004_at
10.5467055122425
200005_at
9.63445800103857
200006_at
10.7655761170168
200007_at
10.7231013205183
200008_s_at
9.54525095552376
200009_at
10.8862511533935
200010_at
10.7627206036558
200011_s_at
9.46337950437173
200012_x_at
11.4733410057858
200013_at
11.3643361459944
200014_s_at
9.6925281212783
200015_s_at
9.92892687511072
200016_x_at
10.5158501791539
200017_at
11.2628055959324
200018_at
11.5397430789843
200019_s_at
10.8077707531688
Total number of rows: 22645 Table truncated, full table size 602 Kbytes .Supplementary file
Size
Download
File type/resource
GSM139904.CEL.gz
2.9 Mb
(ftp) (http)
CEL