|
| Status |
Public on Dec 25, 2006 |
| Title |
DMD skeletal muscle, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
quadriceps muscle tissue of presymptomatic DMD children
|
| Organism |
Homo sapiens |
| Characteristics |
sex: Male
Age: 3 months
additional patient information found in Series supplementary file
|
| Treatment protocol |
Muscle biopsies were flash frozen in liquid nitrogen and stored at -80°C until usage. Samples were homogenized using an IKA ultra-turrax T8 homogenizer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. Tot RNA was further purified using the RNAeasy mini purification kit (QIAGEN) according to the RNA cleanup protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on HG 133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from skeletal muscle tissue of presymptomatic DMD children and age matched controls
|
| Data processing |
The data were analyzed with BRB-Array Tools using rma as probeset summarization algoritm
|
| |
|
| Submission date |
Oct 10, 2006 |
| Last update date |
May 03, 2013 |
| Contact name |
Mario Pescatori |
| E-mail(s) |
[email protected]
|
| Phone |
00393930761013
|
| Organization name |
Health e-genomics
|
| Street address |
Westplein 11
|
| City |
rotterdam |
| ZIP/Postal code |
3016BM |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE6011 |
Expression data from quadriceps muscle of young DMD patients and age matched controls |
|
