total RNA was extracted using Trizol method (Invitrogen)
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
we are interested in which genes are upregulated during T cell anergy. Thus, RNA from normal quiescent T cell clones were extracted to be compared to RNA extracted from anergic T cell clones
