|
| Status |
Public on Apr 29, 2015 |
| Title |
OE21 AZA rep3 |
| Sample type |
RNA |
| |
|
| Source name |
OE21_AZA_24hrs
|
| Organism |
Homo sapiens |
| Characteristics |
cell ine: OE21
tissue origin: Esophagus
cell type: cell line from squamous carcinoma of mid oesophagus
treated with: 50 μM AZA (Azacytidine) for 24 hrs
array id: 9347704059
|
| Treatment protocol |
Het-1A, OE33 and OE22 were treated independently with the HDAC (histone deacetylase) inhibtor MS-275 (0.5 μM), the DNMT1 inhibtor AZA (Azacytidine, 50 μM) and in the combination of both (MS-275 and AZA) for 24 hours. DMSO were used as the respective control. For ech condition was a triplicate performed. After 24 hours stimulation time from all treated samples were the total mRNA isolated.
|
| Growth protocol |
Esophageal cell lines Het-1A, OE21 and OE33 (European Collection of Cell Cultures, Salisbury, UK) were cultured in GIBCO™ RPMI 1640 medium (Invitrogen, Karlsruhe, Germany) supplemented with 10% FBS and 2 mM GIBCO™ L-Glutamine (Invitrogen) at 37 °C in a 5 % CO2 atmosphere
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the QIAGEN RNeasy Plus Mini Kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
OE21_AZA_rep3
|
| Data processing |
All arrays were normalized together using the quantile normalization algorithm without background subtraction using Illumina's BeadStudio Data Analysis Software.
|
| |
|
| Submission date |
Apr 28, 2014 |
| Last update date |
Apr 29, 2015 |
| Contact name |
Hauke Busch |
| E-mail(s) |
[email protected]
|
| Phone |
+49-451-3101-8470
|
| Organization name |
University of Lübeck
|
| Department |
Lübeck Institute of Experimental Dermatology
|
| Street address |
Ratzeburger Allee 160
|
| City |
Lübeck |
| State/province |
Schleswig-Holstein |
| ZIP/Postal code |
23538 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE57130 |
Combination of HDAC inhibitors and Azacytidine for Cancer Cell Selective Targeting of Esophageal Cancer Cells |
|
