|
Status |
Public on Jul 25, 2014 |
Title |
SScMH_32_6mo_RA |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
SScMH_32_6mo_RA
|
Organism |
Homo sapiens |
Characteristics |
tissue: systemic sclerosis human forearm skin biopsy
|
Biomaterial provider |
Northwestern University
|
Treatment protocol |
Storage of human tissue in RNAlater.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA isolated using Qiagen RNeasy Mini Kit.
|
Label |
Cy3
|
Label protocol |
Labeled using Agilent Quick Amp Labeling Kit.
|
|
|
Channel 2 |
Source name |
Stratagene Universal Human Reference
|
Organism |
Homo sapiens |
Characteristics |
sample type: reference
|
Biomaterial provider |
Stratagene
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA isolated by Stratagene.
|
Label |
Cy5
|
Label protocol |
Labeled using Agilent Quick Amp Labeling Kit.
|
|
|
|
Hybridization protocol |
Hybridized using Agilent Hyb Kit.
|
Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000B fluorescent scanner, and image intensity data were extracted and analyzed with GenePix Pro analysis software.
|
Description |
SScMH_32_6mo_RA EXPT_ID: 72498 PRINT_ID: 1064
|
Data processing |
Data for both channels were Lowess-normalized and then the log(2) ratio was taken. Data were filtered for genes with >80% good data.
|
|
|
Submission date |
Apr 17, 2014 |
Last update date |
Jan 14, 2016 |
Contact name |
Tammara Wood |
E-mail(s) |
tammara.a.wood@dartmouth.edu
|
Organization name |
Dartmouth Medical School
|
Department |
Biomedical Data Science
|
Lab |
Whitfield Lab
|
Street address |
WTRB 674
|
City |
Lebanon |
State/province |
NH |
ZIP/Postal code |
03756 |
Country |
USA |
|
|
Platform ID |
GPL6480 |
Series (4)
|
GSE59785 |
Mycophenolate Treatment in Systemic Sclerosis |
GSE59787 |
Hinchcliff 165 microarray dataset |
GSE76809 |
Multi-tissue functional genomic study of systemic sclerosis |
GSE76886 |
Hinchcliff 359 microarray dataset |
|