|
| Status |
Public on Apr 11, 2014 |
| Title |
GzmBKO_HSC 2 |
| Sample type |
RNA |
| |
|
| Source name |
hematopoiteic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
strain: C57BL/6
age: 12 weeks
genotype/variation: GzmB-/-
|
| Growth protocol |
sorted HSCs by flow cytometry
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mRNA was extracted from sorted HSCs and isolated using Arcturus PicoPure (Applied Biosystems).
|
| Label |
biotin
|
| Label protocol |
The NuGEN Ovation Pico WTA System (linear isothermal DNA amplification process) is used to generate single-stranded cDNA from small input amounts of 2.5ng RNA. Labeled cRNA were prepared according to Nugene Encore BiotinIL Module.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridiation protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2, 8294816095_E
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Apr 07, 2014 |
| Last update date |
Apr 11, 2014 |
| Contact name |
Larissa S Carnevalli |
| Organization name |
Astrazeneca
|
| Street address |
Mereside, Alderley Park
|
| City |
Macclesfield |
| State/province |
Cheshire |
| ZIP/Postal code |
SK10 4TG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE56574 |
Gene expression analysis of GzmB-/- hematopoietic stem cells (HSC). |
|
