|
| Status |
Public on May 09, 2014 |
| Title |
FACS sorted CX3CR1hi macrophages from the colons of 6 weeks old CX3CR1gfp/+ mice_SJ38 |
| Sample type |
RNA |
| |
|
| Source name |
FACS sorted CX3CR1hi macrophages_CX3CR1gfp/+
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: wild type; Cx3cr1gfp/+
age: 6 weeks
tissue: colon; lamina propria
cell type: FACS sorted CX3CR1hi macrophages
|
| Treatment protocol |
Cells were sorted from the digested tissue and were sorted using a FACSAria machine (BD).
|
| Growth protocol |
All mice were maintained under specific pathogen-free conditions and handled according to protocols approved by the Weizmann Institute and the Institute Pasteur Animal Care Committee as per international guidelines.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the miRNeasy Mini Kit (Qiagen) for samples 1-2 and with the RNeasy Micro Kit (Qiagen) for samples 3-7. RNA purity was assessed with ND-1000 Nanodrop (Peqlab) and BioAnalyzer 2100 (Agilent).
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix standard protocols
|
| |
|
| Hybridization protocol |
According to Affymetrix standard protocols
|
| Scan protocol |
According to Affymetrix standard protocols, using Affymetrix GeneChip 3000 7G plus scanner
|
| Description |
SJ38_MoST.CEL.pimg
|
| Data processing |
RMA was carried out using Partek Genomic Suite 6.5 (Inc. St. Charles, MO; www.partek.com)
|
| |
|
| Submission date |
Apr 02, 2014 |
| Last update date |
May 09, 2014 |
| Contact name |
Gilgi Friedlander |
| Organization name |
Weizmann Institute of Science
|
| Street address |
Hertzel st
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE56444 |
Macrophage-restricted Interleukin-10 receptor-, but not IL-10 deficiency causes severe spontaneous colitis |
|
