|
| Status |
Public on Sep 18, 2006 |
| Title |
Endometrium in trophoblast conditioned medium for 3 hours TCM-6 |
| Sample type |
RNA |
| |
|
| Source name |
uterus
|
| Organism |
Homo sapiens |
| Characteristics |
Trophoblast conditioned medium endometrium 3 hours TCM-6
|
| Treatment protocol |
E2P4 to decidualization (14 days) + Trophoblast conditionned medium for 3hrs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG 133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner HR3000.
|
| Description |
Trophoblast conditioned medium endometrium 3 hours TCM-6
|
| Data processing |
The data were analyzed with Gene Chip Operating System GCOS version 1.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Sep 14, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Said Talbi |
| E-mail(s) |
[email protected]
|
| Phone |
415-514-2846
|
| Organization name |
UCSF
|
| Street address |
500 Parnassus
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE5809 |
Decidual stromal cell response to paracrine signals from the trophoblast |
|
| Relations |
| Reanalyzed by |
GSE119087 |
