|
| Status |
Public on May 16, 2014 |
| Title |
SAMPLE 22_9423642035_A.raw (7060318066108016723) |
| Sample type |
RNA |
| |
|
| Source name |
ISP thymocytes
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
cell type: immature single positive
cell phenotype: CD4-CD8+CD24+TCRbeta-
|
| Treatment protocol |
Samples were either prepared directly from single cell suspensions or sorted.
|
| Growth protocol |
All studies were performed in vivo.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using RNAzol B (IsoTex Diagnostics) according to the manufacturer’s recommendations and dissolved in RNase free water.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 22
|
| Data processing |
Data were normalized and analyzed with Chipster v2.9: Ilumina normalization for quantile, with consequent log2 transformation of the data
|
| |
|
| Submission date |
Apr 01, 2014 |
| Last update date |
May 16, 2014 |
| Contact name |
Vera C. Martins |
| Organization name |
German Cancer Research Center
|
| Department |
Division of Cellular Immunology
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17543 |
| Series (2) |
| GSE56416 |
Intrathymic origins of T-ALL |
| GSE56419 |
Cell competition is a tumor suppressor mechanism in the thymus. |
|
