|
| Status |
Public on Apr 01, 2014 |
| Title |
miR-17-92_TG1_13.5hr |
| Sample type |
RNA |
| |
|
| Source name |
FoB cells_miR-17-92TG_13.5
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: CD19-Cre;miR-17~92 Tg/Tg mice
cell type: Follicular B cells
stimulation duration: 13.5 hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy kit (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 200ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on the GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 7G.
|
| Data processing |
The data were analyzed with RMA Express using the default settings (Background Adjust - Yes, Normalization - Quantile, Summarization Method - Median Polish, Store Residuals - unchecked).
|
| |
|
| Submission date |
Mar 31, 2014 |
| Last update date |
Apr 01, 2014 |
| Contact name |
Steven Head |
| E-mail(s) |
[email protected]
|
| Phone |
858 784-2263
|
| Organization name |
The Scripps Research Institute
|
| Street address |
10550 N Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL11533 |
| Series (2) |
| GSE56377 |
Follicular B (FoB) cells purified from miR-17-92 transgenic mice, stimulated with LPS/IL-4 for 13.5 or 25.5 hours |
| GSE56379 |
Follicular B (FoB) cells purified from miR-17~92 TG and miR-17~92 tKO mice |
|
