|
| Status |
Public on Sep 13, 2006 |
| Title |
D. simulans/D. mauritiana hybrid adults, D. simulans/D. mauritiana hybrid adults, custom chip, replicate 1, Cy3 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila simulans/D. mauritiana F1 hybrid males, 4 days old
|
| Organism |
Drosophila simulans x Drosophila mauritiana |
| Characteristics |
Drosophila simulans/D. mauritiana F1 hybrids
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was accomplished with Qiagen RNeasy Mini Kit reagents. The RNA (10 µg) was annealed at the Duke Microarray Core Facility.
|
| Label |
Cy3
|
| Label protocol |
The labelling was done at the Duke Microarray Core Facility.
|
| |
|
| Scan protocol |
Fluorescent array images were collected at the Duke Microarray Core Facility.
|
| Description |
D. simulans/D. mauritiana hybrid adults, D. simulans/D. mauritiana hybrid adults, custom chip
|
| Data processing |
The median pixel feature intensity after background subtraction (EXPRESSION column) was log2 transformed, and normalized on Actin5C (giving the VALUE column) to obtain the values given in the data table.
|
| |
|
| Submission date |
Sep 12, 2006 |
| Last update date |
Jun 12, 2013 |
| Contact name |
Amanda J. Moehring |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke University
|
| Department |
DCMB/Biology
|
| Lab |
Mohamed Noor
|
| Street address |
Box 91000
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL4022 |
| Series (2) |
| GSE5599 |
Comparison of D. simulans/D. mauritiana F1 male hybrid custom expression values to pure-species parents |
| GSE5655 |
Comparison of pure-species Drosophila expression to hybrid expression |
|
