|
| Status |
Public on Mar 20, 2014 |
| Title |
Tr1_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
in vitro differentiated murine Th cell subset; Tr1
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
cell type: sorted naive Th cells (CD4+ CD44- Foxp3-)
differentiated with: 100 ng/ml IL-27 plus 0.5 ng/ml TGF-β
th cell subtype: Tr1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis was performed using the AmbionR WT expression kit. Single-stranded cDNA was fragmented and labelled with biotin allonamide triphosphate using the GeneChipR WT terminal labelling kit.
|
| |
|
| Hybridization protocol |
5µg cDNA were hybridized to Affymetrix Mouse Gene 1.0 ST arrays (Hybridization, Wash and Stain Kit Affymetrix)
|
| Scan protocol |
Affymetrix scanner 3000 7g.
|
| Description |
Gene expression data Tr1
|
| Data processing |
affymetrix-algorithm-name = rma-gene-default (v.1.0)
program-name = Affymetrix Expression Console (v.1.3.1.187)
|
| |
|
| Submission date |
Mar 19, 2014 |
| Last update date |
Mar 20, 2014 |
| Contact name |
Thomas Korn |
| Organization name |
TU Munich, Klinikum rechts der Isar
|
| Department |
Neurology
|
| Lab |
Neuroimmunology
|
| Street address |
Ismaninger Str. 22
|
| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE56021 |
in vitro differentiated Th0, Th17, and Tr1 cells |
|
