|
| Status |
Public on Aug 20, 2015 |
| Title |
Donor 1 Tx.7hrs.2 |
| Sample type |
RNA |
| |
|
| Source name |
Dendritic cell
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Tx
cell type: Dendritic cell
time (hours): 7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the RNeasyPLUS kit (Qiagen) according to the manufacture’s instructions
|
| Label |
biotin
|
| Label protocol |
Preparation of cDNA, cRNA, and labeling were carried out by the Yale Center for Genomic Analysis using the standard Illumina protocols. Hybridization buffer from the BeadChip kit (Illumina) was mixed with 1500 ng of biotin-labeled cRNA, heated to 65°C for 5 minutes, and then loaded onto the BeadChip. The BeadChips were sealed in a hybridization chamber and placed in an oven at 58°C with a rocker for 16-20 hours. After the hybridization, the BeadChips were washed and stained in a series of washes and stains as outlined in the Illumina protocol. The BeadChips were scanned on the Illumina Iscan.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Dendritic cell was treated by Tx for 7 Hours
|
| Data processing |
The data were median normalized
|
| |
|
| Submission date |
Feb 22, 2014 |
| Last update date |
Aug 20, 2015 |
| Contact name |
Hailong Meng |
| Organization name |
Yale University
|
| Street address |
300 George Str
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06525 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE55276 |
Temporal Response to seasonal and pandemic H1N1 infection in human DCs- Donor 1 |
| GSE55278 |
Temporal Response to seasonal and pandemic H1N1 infection in human DCs |
|
