|
| Status |
Public on Sep 19, 2006 |
| Title |
DC3 RSG AGN |
| Sample type |
RNA |
| |
|
| Source name |
Monocyte derived dendritic cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 92 kg
|
| Treatment protocol |
Cells were treated with 2.5 uM rosiglitazone (RSG) along with 1 uM AGN193109 (AGN). Ligands or vehicle were added to the cell culture starting from the first day. AGN193109 administration was repeated at day 3.
|
| Growth protocol |
Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
|
| Label |
biotin
|
| Label protocol |
cRNA was generated from 5 ug of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit.
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Data processing |
Affymetrix GCOS 1.4, statistical (MAS5) algorithm
|
| |
|
| Submission date |
Aug 31, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Istvan Szatmari |
| E-mail(s) |
[email protected]
|
| Phone |
36 52 416-432
|
| Fax |
36 52 314-989
|
| Organization name |
University of Debrecen
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Lab |
Nuclear Hormone Receptor
|
| Street address |
Egyetem tér 1.
|
| City |
Debrecen |
| ZIP/Postal code |
H-4010 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE5679 |
Comparative gene expression profile of PPARg and RARa ligand treated human dendritic cells |
|
| Relations |
| Reanalyzed by |
GSE49910 |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
