|
| Status |
Public on Jan 20, 2015 |
| Title |
GSK126.2 |
| Sample type |
RNA |
| |
|
| Source name |
OVISE cell line, GSK126 treated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ARID1A mutated clear cell ovarian cancer OVISE
condition: EZH2 inhibited
batch: batch 2
|
| Treatment protocol |
Matrigel media was treated with either vehicle control DMSO, 1 ug/mL doxycycline to induce wild type ARID1A expression or 5μM GSK126 to inhibit EZH2
|
| Growth protocol |
ARID1A mutated clear cell ovarian cancer OVISE cells restored for wild type ARID1A expression or treated with 5μM GSK126 were cultured in 3D using Matrigel.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction using trizol, Rneasy clean-up and Dnase I treated
|
| Label |
biotin
|
| Label protocol |
Biotin Labeling of RNA at 400ng with Ambion Illumina® TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Hybridized on Illumina Human-HT12 v4 Expression BeadChip Kit using Illumina whole-Genome Gene Exprssion with Intellihyb Seal system which detect signal with streptavidin-Cy3
|
| Scan protocol |
Illumina Setting with PMTFactor=2
|
| Description |
inhibition of EZH2 by GSK126
SAMPLE 8
|
| Data processing |
Illumina GenomeStudio software was used to export expression levels and detection p-values for each probe of each sample. Signal intensity data was quantile normalized and genes that showed insignificant detection p-value (p>0.05) in all samples were removed from further analysis.
|
| |
|
| Submission date |
Feb 13, 2014 |
| Last update date |
Jan 20, 2015 |
| Contact name |
Louise C Showe |
| E-mail(s) |
[email protected]
|
| Phone |
215-898-3791
|
| Organization name |
The Wistar Institute
|
| Lab |
Dr. Louise Showe
|
| Street address |
3601 Spruce St
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE54979 |
Targeting EZH2 methyltransferase activity in ARID1A mutated cells as a synthetic lethal therapeutic strategy |
|
