|
| Status |
Public on Feb 06, 2014 |
| Title |
Nr4a1 kidney at week8, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney isolated from Nr4a1-/- at week 8
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: kidney
genotype: point mutation in exon 1 of Nr4a1 gene
age: 8 weeks of age
|
| Treatment protocol |
Whole genome transcript analysis was performed using Affymetrix platform on kidney tissue collected from FHH and Nr4a1-/- at week 8 (n=3 per group), 16 (n=3), and 24(n=3).
|
| Growth protocol |
FHH and Nr4a1-/- rats were grown to 8, 16, and 24 weeks of age and kidney tissue was harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using TRIzol® and Invitrogen PureLink Kit (Life Technologies) and evaluated for quality and integrity (Bio-Rad Experion™ System)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol for Rat GeneChip 1.0ST Arrays
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized to Rat GeneChip 1.0ST Arrays. GeneChips were automatically washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G system
|
| Description |
Gene expression data from Nr4a1-/- kidney at 8 weeks of age
|
| Data processing |
Analysis of microarray data was performed using software provided by Affymetrix (Affymetrix® Expression Console™ Software) and commercially available GeneSifter™ software platform. Microarrays were normalized using RMA alogrithm.
affymetrix-algorithm-name = rma-gene-default
affymetrix-algorithm-version = 1.0
affymetrix-array-type = RaGene-1_0-st-v1
program-name = Expression Console
program-version = 1.1.2800.28061
program-company = Affymetrix
affymetrix-algorithm-param-apt-engine = ProbesetSummarizeEngine
affymetrix-algorithm-param-apt-prog = Expression Console
|
| |
|
| Submission date |
Feb 05, 2014 |
| Last update date |
Feb 06, 2014 |
| Contact name |
Michael R Garrett |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Mississippi Medical Center
|
| Department |
Pharmacology
|
| Street address |
2500 North State Street
|
| City |
Jackson |
| State/province |
MS |
| ZIP/Postal code |
39110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE54714 |
Gene Expression of Kidney from FHH and Nr4a1 Deficient Rats |
|
